Abstract
Suppression of pathogenic bacterial growth to increase food and agricultural productivity is important. We previously developed novel hexapeptides (KCM12 and KCM21) with antimicrobial activities against various phytopathogenic bacteria and N2 plasma-treated buffer (NPB) as an alternative method for bacterial inactivation and as an antibiofilm agent of crops. Here, we developed an enhanced antibiofilm method based on antimicrobial hexapeptides with N2 plasma-treated buffer against plant pathogens. Our results demonstrated that hexapeptides effectively inhibited the growth of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and the biofilm it formed. Potent biofilm formation-inhibiting effects of hexapeptides were observed at concentrations of above 20 µM, and samples treated with hexapeptide above 100 µM reduced the ability of the bacteria to produce biofilm by 80%. 3D confocal laser scanning microscopy imaging data revealed that the antimicrobial activity of hexapeptides was enough to affect the cells embedded inside the biofilm. Finally, combination treatment with NPB and antimicrobial hexapeptides increased the antibiofilm effect compared with the effect of single processing against multilayered plant pathogen biofilms. These findings show that the combination of hexapeptides and NPB can be potentially applied for improving crop production.
Highlights
Controlling biofilm formation by numerous plant-related pathogenic bacteria is one of the most difficult challenges faced when increasing the production of high-quality crops
Our results show that using antimicrobial hexapeptides in combination with N2 plasma-treated buffer (NPB) has an excellent antibiofilm effect against multilayered plant pathogen biofilms, and that the penetration efficacy of NPB into multilayered biofilms is one of the foremost vital properties contributing to its strong antibiofilm activity
We proved that the novel hexapeptides, KCM12 and KCM21, exhibit broad-spectrum considered to be a promising alternative to previous antibiotics [18,19]
Summary
Controlling biofilm formation by numerous plant-related pathogenic bacteria is one of the most difficult challenges faced when increasing the production of high-quality crops. Among the various methods used, antibiotics are one of the most important antimicrobial substances used in the treatment and prevention of bacterial infections. We previously developed N2 plasma-treated buffer (NPB) as an alternative method for bacterial inactivation and as an antibiofilm agent of crops. In this study, we developed an enhanced antibiofilm method on the basis of antimicrobial hexapeptides with NPB against the plant pathogen Pst DC3000. The effect of combination treatment of hexapeptides with NPB was increased in controlling pathogenic bacteria. Our results show that using antimicrobial hexapeptides in combination with NPB has an excellent antibiofilm effect against multilayered plant pathogen biofilms, and that the penetration efficacy of NPB into multilayered biofilms is one of the foremost vital properties contributing to its strong antibiofilm activity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
