Abstract
Phytochemicals are chemical compounds often referred to as secondary metabolites. Twenty eight bioactive phytochemical compounds were identified in the methanolic extract of Nigella sativa. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, and molecular formula. Gas chromatography-mass spectrometry (GC-MS) analysis of Nigella sativa revealed the existence of the s-Pinene, D-Glucose, 6-O-α-Dgalactopyranosyl, O-Cymene, DL-Arabinose, Trans-4-methoxy thujane, 2-Propyl-tetrahydropyran-3-ol, Terpinen-4-ol, α- D-Glucopyranoside, O-α-D-glucopyranosyl-(1.fwdarw.3)-s-D-fruc, Thymoquinone, 2-Isopropylidene-5-methylhex-4-enal, Limonen-6-ol, pivalate, Longifolene, 2-(4-Nitrobutyryl)cyclooctanone, s-Bisabolene, 1,1-Diphenyl-4-phenylthiobut-3-en-1-ol, Phenol, 4-methoxy-2,3,6-trimethyl, Pyrrolidin-2-one-3s-(propanoic acid, methyl ester),5-methylene-4α, Cholestan-3-ol, 2-methylene-,(3s,5α), l-(+)-Ascorbic acid 2,6-dihexadecanoate, 9,12-Octadecadienoic acid (Z,Z)-, methyl ester, 1-Heptatriacotanol, 10,13-Eicosadienoic acid, methyl ester, E,E,Z-1,3,12-Nonadecatriene-5,14-diol, 9-Octadecenamide,(Z), 2H-Benzo[f]oxireno[2,3-E]benzofuran-8(9H)-one,9-[2-(dimethylar, Phthalic acid, decyl oct-3-yl ester, 1,2-Benzenedicarboxylic acid, bis(8-methylnonyl) ester and Stiqmasterol. Key words: Gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, Nigella sativa, phytochemicals.
Highlights
Nigella sativa L. (Ranunculaceae) is an annual herbaceous plant native to Southwest Asia, and cultivated and naturalized in Europe and North Africa (Al-Johar et al, 2008)
N. sativa seeds are washed with water, dried and ground into powder
The filtrates were used for further phytochemical analysis
Summary
Nigella sativa L. (Ranunculaceae) is an annual herbaceous plant native to (and cultivated in) Southwest Asia, and cultivated and naturalized in Europe and North Africa (Al-Johar et al, 2008). A member of Ranunculaceae family, known as the black cumin seeds is one of the most revered medicinal seeds in history. It is an annual aromatic plant and its cultivation is traced back more than 3000 to the kingdom of the Assyrians and ancient Egyptians (Ashraf et al, 2006; Hameed et al, 2015a). Seeds of N. sativa are reported to contain amino acids, carbohydrates, fixed and volatile oils. The extracts of N. sativa (Black seeds) have been used by patients to suppress coughs, disintegrate renal calculi (Hashem and El-Kiey, 1982), retard the carcinogenic process, treat abdominal pain, diarrhea, flatulence, and polio (Enomoto et al, 2001), exert choleretic and uricosuric activities, anti-inflammatory and antioxidant effects (Mansour et al, 2002; Altameme et al, 2015b). The essential oil was shown to have antihelminthic, antischistosomal (Mahmoud et al, 2002), antimicrobial (Aboul-Ela et al, 1996), and antiviral
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