Abstract
The study is essential in reducing germination time of Treculia africana var. inversa. The effect of three different concentrations of sucrose namely 2, 3 and 4% were investigated on the in vitro regeneration of embryo explants of T. africana Decne. on the media of Murashige and Skoog (MS) and Gamborg et al. (B5) respectively without any growth regulator. The experimental design was a 2 × 5 factorial in a completely randomized design with each treatment consisting of ten replicates. Results showed that while both media including control (contains agar only) supported the in vitro regeneration of T. africana embryo explants, B5 medium was found to be significantly superior (P≤0.05) to MS medium in all the growth parameters studied. B5 medium at 4% sucrose elicited the best response in all the growth parameters determined while control gave the least response. The protocol reported here can be used for large scale propagation of true-to-type T. africana plants within a short time for the purpose of improvement through genetic transformation (mutagenesis) and the development of a viable conservation programme. Key words: Treculia africana, Murashige and Skoog (MS) medium and Gamborg et al. (B5) medium, embryo explant.
Highlights
IntroductionTreculia africana Decne. (commonly known as African bread fruit, Wild jack fruit, or African boxwood), is an important multipurpose indigenous tree species in West Africa belonging to the family Moraceae (Nutrecul Agroforestry Company (NAC), 2013)
Treculia africana Decne., is an important multipurpose indigenous tree species in West Africa belonging to the family Moraceae (Nutrecul Agroforestry Company (NAC), 2013)
The embryos (Figure 3) of T. africana cultured on both Murashige and Skoog (MS) and B5 media began to show visible changes by the 2nd day in culture
Summary
Treculia africana Decne. (commonly known as African bread fruit, Wild jack fruit, or African boxwood), is an important multipurpose indigenous tree species in West Africa belonging to the family Moraceae (Nutrecul Agroforestry Company (NAC), 2013). (commonly known as African bread fruit, Wild jack fruit, or African boxwood), is an important multipurpose indigenous tree species in West Africa belonging to the family Moraceae (Nutrecul Agroforestry Company (NAC), 2013). It is a monoecious dicotyledonous plant with flowers crowded into compact heads (Ugwoke et al, 2003). T. africana is a large, evergreen tree growing in the forest up to 30 m high with a girth of 4-6 m (Agbogidi and Onomeregbor, 2008) It has a dense spreading crown and fluted trunk. Their taxonomic differences are based mainly on the size of the fruit head (infructence) and the hairiness of the branchlets and leaves (NAC, 2013)
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