Abstract
Senna alata L. Roxb, is an ethnomedicinal plant belonging to Fabaceae family. The plant is known to possess potent medicinal properties include laxative, antitumor, antimutagenic, antioxidant and antifungal. Seeds of S. alata possess a hard seed coat which acts as a barrier for seed germination. Zygotic embryos were cultured on various media (half and full - strength Murashige and Skoog's medium, Gamborg's medium) with different concentrations of sucrose(10-30g/L) and plant growth regulators (PGRs). Maximum percentage of embryo germination and healthy plantlet formation were observed on MSO (Murashige and Skoog basal) medium containing 20 g/L sucrose without PGRs. Multiple shoot formation was observed on MS (Murashige and Skoog's) medium containing 20g/L sucrose supplemented with 2 mg/L BAP. The formed seedlings were acclimatized in sterile vermiculite: garden soil (1:1) and later shifted to field conditions. The newly formed plantlets resembled the donor plant phenotypically. This protocol can be used for multiplication and conservation of the species.
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