Abstract
The application of bacteriophages biocontrol requires the formulation of genetically distinct bacteriophages in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered a cheap, reproducible, and readily applicable tool in detecting phage diversity compared to other molecular techniques such as whole-genome sequencing. We used in this study the RAPD-PCR technique to assess the genetic diversity of 28 bacteriophages infecting Pseudomonas aeruginosa and Staphylococcus aureus. According to their RAPD profiles, isolated phages were grouped into 2 main clusters which included phages from the same host. The typing by RAPD-PCR of newly isolated phages was useful to assess the genetic diversity bypassing previous whole-genome sequencing analysis. These genetically distinct phages lytic against P. aeruginosa and S. aureus could potentially be used in a phage cocktail for biocontrol against these clinically and industrially relevant bacteria. Key words: Phages, genetic diversity, RAPD PCR, P. aeruginosa, S. aureus.
Highlights
Bacteriophages, the viruses of bacteria are the most abundant biological entities in the biosphere and are always associated with their host bacteria in the same ecological niche
Phages with lytic activity against these bacterial pathogens are sourced from the environment or clinical specimens linked to diseases caused by P. aeruginosa and S. aureus
We investigate lytic phages against P. aeruginosa and S. aureus specific-phages
Summary
Bacteriophages (phages), the viruses of bacteria are the most abundant biological entities in the biosphere and are always associated with their host bacteria in the same ecological niche. Phages have an impact on the bacterial ecosystems both locally in our immediate communities and globally (Abedon, 2008; de Leeuw et al, 2020; Harada et al, 2018) Both Pseudomonas aeruginosa and Staphylococcus aureus are virulent pathogenic organisms for many clinical conditions. S. aureus is responsible for skin and soft tissues infections and invasive diseases such as endocarditis, osteomyelitis (Wang et al, 2016). Both species are notorious for their resistance to antimicrobials. Phages with lytic activity against these bacterial pathogens are sourced from the environment or clinical specimens linked to diseases caused by P. aeruginosa and S. aureus. The RAPD PCR of isolated phage DNA extracts helped to discriminate between the phages and to assess their genetic diversity
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