Abstract
Ornithobacterium rhinotracheale has been identified as a newly emerging respiratory bacterial pathogen that has been causing significant economic losses to the poultry industry. In this study, a total of 30 isolates of O. rhinotracheale isolated (2 from layer pullets, 5 from broilers, 21 from turkeys and 2 standart strains) from chickens and turkeys were characterized by Polymerase Chain Reaction (PCR), RAPD-PCR and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis. The isolates of O. rhinotracheale identified by biochemical tests were also confirmed by PCR. Amplification product of 784 bp was obtained, which is corresponding to the expected size. All of them were characterized as O. rhinotracheale. The random amplified polymorphic DNA (RAPD) profiles using OPG-11 primer and the protein profiles used in SDS-PAGE of the O. rhinotracheale strains show high variability in results of Neighbor Joining (NJ) and Unweighted Pair Group Method with Arithmetic Means (UPGMA). All field strains isolated from turkeys, broilers and layers show different RAPD and protein profiles. Key words: Ornithobacterium rhinotracheale, broiler, layer, turkey, polymerase chain reaction (PCR), random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR), sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE).
Highlights
Ornithobacterium rhinotracheale is an infectious agent which has an aetiologic role in the respiratory disease complex in poultry (van Beek, 1994; Vandamme et al, 1994; Hafez, 1996; Chin and Droual, 1997)
A total of 30 isolates of O. rhinotracheale isolated (2 from layer pullets, 5 from broilers, 21 from turkeys and 2 standart strains) from chickens and turkeys were characterized by Polymerase Chain Reaction (PCR), random amplified polymorphic DNA (RAPD)-PCR and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis
The 21 O. rhinotracheale strains were isolated from 21 turkey flocks that were suffering respiratory diseases
Summary
Ornithobacterium rhinotracheale is an infectious agent which has an aetiologic role in the respiratory disease complex in poultry (van Beek, 1994; Vandamme et al, 1994; Hafez, 1996; Chin and Droual, 1997). O. rhinotracheale can be a primary or secondary cause which depends on strain virulence, adverse environmental factors (poor management, inadequate ventilation, high stocking density, poor litter condition, poor hygiene, high levels of respiratory ammonia), immunity state of flock, and presence of other infectious agents. The primary role of O. rhinotracheale in respiratory disease is open to debate (De Rosa et al, 1996; Erganis et al 83 van Veen et al, 2000; Erganis et al, 2002; Hadimli et al, 2003; Canal et al, 2005)
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