Abstract
Random amplified polymorphic DNA (RAPD) markers were used to assess relationship across nine aquatic species of Utricularia. The highest numbers of RAPD bands were detected in Utricularia bremii and U. intermedia. The highest genetic similarity was observed between U. australis and U. dimorphantha; between U. australis and U. vulgaris; and between U. dimorphantha and U. macrorhiza indicating that these species are closely related. Unweighted pair group method with arithmetic mean (UPGMA) analysis based on the RAPD profile in aquatic Utricularia resolved the taxa into three clusters: the first cluster included U. aurea, U. australis, U. dimorphantha, U. macrorhiza and U. vulgaris; the second cluster constituted from U. bremii and U. gibba while U. intermedia formed the third cluster with U. minor. The Neighbour Joining (NJ) tree showed that aquatic species could be placed in two main groups and the results obtained from the NJ analysis were coherent with that of the UPGMA clustering method. The molecular findings revealed from this study support the systematic relationships in Utricularia inferred from morphological investigations. Key words: Aquatic Utricularia, Genetic affinity, Neighbour Joining, RAPD, Systematic relationships, UPGMA DOI: 10.3329/bjpt.v13i2.580 Bangladesh J. Plant Taxon. 13(2): 73-82, 2006 (December)
Highlights
The development of random amplified polymorphic DNA (RAPD) markers generated by polymerase chain reaction (PCR) using arbitrary primers has resulted in alternative molecular markers for the detection of nuclear DNA polymorphism (Williams et al 1990)
The present study offers a methodological approach using RAPDs to investigate partitioning of variation and taxonomic relationships among nine aquatic species of Utricularia
The highest number of RAPD bands was observed in Utricularia intermedia followed by U. bremii
Summary
The development of random amplified polymorphic DNA (RAPD) markers generated by polymerase chain reaction (PCR) using arbitrary primers has resulted in alternative molecular markers for the detection of nuclear DNA polymorphism (Williams et al 1990). The RAPD technique has many advantages such as detection of polymorphism, relatively inexpensive, fast, and reliable It utilizes primers of arbitrary sequences which may be used for different species and does not require previous knowledge of DNA sequence. The present study offers a methodological approach using RAPDs to investigate partitioning of variation and taxonomic relationships among nine aquatic species of Utricularia. This investigation examines the suitability of RAPDs as a tool for identifying Utricularia species and detecting genetic variability among the species
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