Abstract
This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the development of adventitious roots was liquid MS media supplemented with 40 mg/L sucrose and maintained at pH 6.0. Key words: Adventitious root, stem, basic MS media.
Highlights
Panax bipinnatifidus SEEM. (Araliaceae) is a hygrophilous and shade-enduring plant, preferring cool and wet climate conditions with an average temperature of about 12-15°C
Results from ANOVA table show that there is no significant difference between the effects of indolebutyric acid (IBA) and NAA
On the callus induction efficiency (P>0.001); there was a significant difference of the D treatment (2,4-D) (F=127.6 với p
Summary
Panax bipinnatifidus SEEM. (Araliaceae) is a hygrophilous and shade-enduring plant, preferring cool and wet climate conditions with an average temperature of about 12-15°C. (Araliaceae) is a hygrophilous and shade-enduring plant, preferring cool and wet climate conditions with an average temperature of about 12-15°C. In nature, this plant is relatively rare and mostly found in the high mountainous region of. Are characterized, including compounds in oleanolic triterpenoid saponin group: Bifinoside A-C (1-3), narcissiflorine methyl ester (4), chikusetsu saponin IVa (5), pseudoginsenoside RP1 methyl ester (6), stipuleanoside R1 (7), pseudoginsenoside. RT1 methyl ester (8), momordinIIe (9) and stipuleanoside. R2 methyl ester (10) (Tung et al, 2011). P. bipinnatifidus roots contain saponins triterpens (chikusetsu saponin IV, zingibrosid R1, ginseninosid Ro, Rb, Rd, Re, Rg1, Rg2,...), and phytosterol, reducing sugars, oils, uronic acids and fatty acids. Extracts from P. bipinnatifidus has metabolic activity (Dua et al, 1989), anti-inflammatory activity (Matsuda et al, 1991), anti-oxidation
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