Abstract
The endonucleolytic activity from an extract of Micrococcus luteus is used to detect small amounts of damage in the DNA of bacteria exposed to ultraviolet light or γ rays. The extract is added directly to a lysate of the bacteria, and after a short period of incubation a sample of the bacterial DNA is assayed for endonuclease-sensitive sites by sedimentation through alkaline sucrose gradients. As few as one or two sites per 10 8 daltons of DNA can be detected by this method.
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