Encoding gene mutation of mitochondrial DNA in esophageal cancer cells

Abstract

Objective To investigate the relationship of mitochondrial DNA (mtDNA) encoding gene mutation with the occurrence and development of esophageal cancer. Methods MtDNA was extracted in three kinds of human esophageal cancer cell lines EC9706, TE-1 and Eca109, and normal esophagus cell line, Het-1A. All of the encoding genes were sequenced. Results There were 39 mutations in three kinds of esophageal cancer cells, and 11 mutations in Het-1A cells. Most of the mutations were A-G or C-T. A total of 11 protein coding mutations have been identified. Except A8860G and A15326G, the two mutations appeared in normal esophageal cells at the same time, and the remaining 9 mutations only appeared in cancer cells (G6366A, A8701G, A10086G, A10398G, A12358G, A13105G, C14766T, A15311G, and A15824G). The corresponding mutation genes were cytochrome c oxidase subunit 1 (COX1), ATP synthase 6 (ATP6), nicotinamide adenine dinucleotide dehydrogenase 3 (ND3), ND3, nicotinamide adenine dinucleotide dehydrogenase 5 (ND5), ND5, cytochromes B (CytB), CytB, CytB. Conclusion The mutations of the mtDNA encoding gene of esophageal cancer cells were related to the occurrence and development of esophageal cancer. Key words: Esophageal cancer; Mitochondrial DNA; Encoding gene; Mutation