Expression of miRNA-22 in esophageal and gastric cancer tissues and cell lines

Abstract

Objective To investigate the expression and clinical value of miRNA-22 in esophageal and gastric cancer tissues and cell lines. Methods Realtime-PCR was performed to detect the expression of miRNA-22 in human esophageal cancer cell lines ECA109, TE-1, TE-8, TE-13 and normal esophageal epithelial cells HEEC, 48 cases of esophageal cancer tissues and matched adjacent normal tissues, human gastric cancer cell lines SGC-7901, MKN-45, NCI-N87, AGS, NUGC-3, SUN-1, normal human gastric mucosa cell line GES-1 and normal stomach fibroblastic cell NSFC, 88 cases of gastric cancer tissues and matched adjacent normal tissues. Results The expression of miRNA-22 was much less in four esophageal cancer cell lines than that of immortalized normal esophageal epithelial cells HEEC, and the expression of miRNA-22 was much less in six gastric cancer cell lines than that of gastric mucosal epithelial cell line GES-1 and normal stomach fibroblastic cell line NSFC.The esophageal cancer tissues showed aberrant down regulation of miRNA-22 compared with the adjacent non-tumor tissues [(3.51±1.05) vs.(11.23±2.95), t=18.13, P<0.05]. The gastric cancer tissues showed aberrant down regulation of miRNA-22 compared with the adjacent non-tumor tissues [(2.15±1.23) vs.(9.14±2.86), t=22.93, P<0.05]. Conclusion The expression of miRNA-22 was much lower in esophageal and gastric cancer tissues and cell lines, which provided basement to nosogenesis, early diagnosis and create drug treatment of the cancers. Key words: Esophageal neoplasms; Gastric neoplasms; Cell lines; microRNA-22