Empirical equation for the accurate prediction of retention in planar chromatography

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Either a second order or a third polynomial equation accurately predicts R F in a mobile phase which is a binary mixture of a strong and weak solvent. The mole fraction of the strong solvent is used as the independent variable. The p-nitrobenzyl esters of fifteen dansyl amino acids were used as model solutes for planar chromatography, using each of five different mobile phases on silica gel layers. For either of the polynomial equations, a minimum of 98% of the predicted R F values, at mole fractions not used for establishing the equations, are within 0.05 or less of an R F unit (when compared to the experimental R F ), and 90% of the predictions are within 0.03 or less of an R F unit.