Abstract
The experimental profiles obtained for large size samples in normal- and reversed-phase liquid chromatography are compared to the profiles predicted by the theory of nonlinear chromatography. When the sorption isotherms of the analytes are determined accurately, the agreement between the experimental and the theoretical profiles is quantitative in all cases. When the solvent is a mixture of a strong and a weak solvent, and if a nonselective detector is used, both the competitive adsorption isotherms of the analyte and the strong solvent should be taken into account. Then a two-component model of nonlinear chromatography must be used, and the system peaks are predicted. If a nonselective detector is used, however, which gives no response for the solvent (i.e., no system peak is detected), and the strong solvent is less strongly adsorbed than the solute studied, the elution profile of a large concentration band can be predicted with a one-compound model, knowing the adsorption isotherm of the solute from the solution. The conditions under which a single-component model can be used to predict with good or excellent agreement the profiles of large concentration bands of analyte eluted with a binary mobile phase are investigated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
