ELISA diagnostic test systems, serodiagnosis of arboviral infections

Abstract

There are developed and tested by ourselves ELISA kits for serodiagnostics of most endemic to Russia arboviral infections and some imported tropical diseases. Their application allowed in 1999 to set the value WN virus in the etiology of outbreaks ofWN fever in the southern region of Russia, then in Voronezh and Tula regions. From 1999 to 2014, according to the data of Russian Service for Surveillance on Consumer Rights Protection and Human Wellbeing in the Russia there were diagnosed 2284 cases of WNfevers and 1725 cases of CCHF. In 2012-2014 specific IgG and neutralizing antibodies to the WN virus were detected in the population of the Southern and Black earth regions of Russia. Data of the examination of residents ’ sera from Tatarstan, Tver, Ryazan, Kaluga, Kursk and Vologda regions were proved to be negative. In 2000 there were revealedfirst cases of CCHF in the Volgograd region, Kalmykia and Dagestan also. The application of ELISA-IgM kits allowed to reveal cases of Sindbis, Batai, Tahyna, Inkoo, sandfly fevers, Uukuniemi and Bhanja. During the period of2009-2014 there were verified 190 imported cases of dengue fever, Chikungunya, WNfever, sandfly fevers, and Japanese encephalitis among persons returning from travel in tropical countries.