Abstract
Polyacrylonitrile fibers with and without magnetic nanoparticles (Fe3O4 NPs) were prepared by electrospinning. The pure polyacrylonitrile (PAN) fibers and the composited polyacrylonitrile (PAN/Fe3O4) fibers were studied with respect to their capability for enrichment of glycoproteins. Specifically, the glycoproteins ovalbumin (OB) and transferrin (Trf) were studied and compared to the non-glycoproteins bovine serum albumin and lysozyme. Following adsorption and subsequent protein elution with 0.1wt% of CTAB solution, the glycoproteins were analyzed by SDS polyacrylamide gel electrophoresis. The strong interaction between PAN or PAN/Fe3O4 fibers and glycoproteins is attributed to the synergistic effects of hydrophilic and hydrogen bond interactions. The PAN/Fe3O4 fibers have an attractive additional feature of allowing magnetic separation. The PAN and PAN/Fe3O4 fibers have a high adsorption capacity toward OB and Trf. The treated PAN/Fe3O4 fibers display good selectivity, fast adsorption kinetics, and were applied to extractions of mixed protein samples. The detection limits of OB and Trf are 0.32 and 0.22μg·mL-1, respectively. The PAN/Fe3O4 fibers offered an alternative solution for adsorption of glycoproteins from biological samples. Graphical abstract The pure polyacrylonitrile (PAN) fibers and the composited polyacrylonitrile (PAN/Fe3O4) fibers were studied with respect to their capability for enrichment of glycoproteins: glycoproteins ovalbumin (OB) and transferrin (Trf). The treated PAN/Fe3O4 fibers showed fast adsorption kinetics, were applied in a physiological state, mixed and real samples.
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