Abstract
Previous studies in this department with 2-ethyl-2-phenyl butyramide (EPB) have shown that this compound is extremely hepatotoxic in dogs and produces precipitation of yellowish-brown material in hepatocytes and bile canaliculi. Continuous investigations have established that the hepatic injury induced by EPB is porphyria.EPB was administered orally in capsules to male dogs and to both pregnant and non-pregnant female dogs for periods ranging from 10 to 42 days at a total daily dose level of 50 mg/kg/day.Light microscopic examination of sections of liver from adult dogs as well as newborn animals from treated mothers showed prominent yellowish-brown deposits in the hepatocytes, bile canaliculi, and sinusoids. These deposits closely resembled the bile plugs commonly seen in cholestasis. However, histochemical tests showed that these “pseudo” plugs contained neither conjugated nor unconjugated bilirubin.Samples of serum, urine, and bile, as well as slices of liver of dogs given EPB, showed red fluorescence when subjected to ultra-violet light, indicating the presence of porphyrin.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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