Electric pulse stimulation inhibited lipid accumulation on C2C12 myotubes incubated with oleic acid and palmitic acid

Abstract

Objective To investigate the effect of electrical pulse stimulation (EPS) on lipid accumulation and alteration of fatty acid-related enzymes in C2C12 myotubes incubated with fatty acids. Methods Mouse C2C12 myotubes were incubated with oleic acid and palmitic acid, and differentiated C2C12 myotubes were treated with EPS, oil-red O (ORO), BODIPY staining and triglyceride (TG) content were examined. Total RNA was isolated, and real-time polymerase chain reaction analysis was performed. Results (1) EPS decreased TG content (p < .01). (2) EPS significantly induced the mRNA expression of FAD/CD36 (p < .05), FATP4 (p < .001), FABP1 (p < .01) and FABP5 (p < .01). (3) EPS significantly inhibited the mRNA expression of fatty acid synthase (p < .01). (4) Adipose triglyceride lipase and hormone-sensitive lipase expression were significantly elevated (p < .001), and induced the mRNA expression of CPT1 (p < .01), ACOX1 (p < .05), UCP3 (p < .05) and PPARα (p < .001) after EPS. Conclusion EPS reduced lipid droplet accumulation; enhanced CD36, FATP4, FABP1 and FABP5 expression; inhibited C2C12 myotube fatty acid re-esterification; and promoted fatty acid oxidation in C2C12 myotubes.