Electric field stimulation protects injured spinal cord from secondary inflammatory response in rats.

Abstract

To investigate acute beneficial effects of electrical field stimulation (EFS) on secondary inflammatory response in spinal cord injury (SCI) rats. Sprague-Dawley (SD) rats were divided into three groups,sham group rats received laminectomy only, control group rats were subjected to SCI only, and EFS group rats received EFS immediately after the injury. During the 30-min-stimulation, the injury potential modulated to 0 ± 0.5 mV by EFS. At 12h, 24h and 48h after the surgery, the rats in each group were sacrificed. Immunofluorescence staining for macrophage marker (ED-1), the tautomerase activity of macrophage inhibitory factor (MIF) assay and real-time PCR analysis for interleukin-1β (IL-1β) and matrix metalloproteinase-9 (MMP-9) were performed. Compared to the rats in control group, the rats treated with EFS presented less ED-1 positive cells 12h (P <; 0.05), 24h (P <; 0.01) and 48h (P <; 0.05) after the surgery and showed a lower MIF tautomerase activity 12h (P <; 0.01), 24h (P <; 0.01) and 48h (P <; 0.01) after the surgery. Moreover, EFS significantly decreased the mRNA levels of IL-β (P <; 0.05) and MMP-9 at 48h (P <; 0.01) after the injury. EFS could attenuate secondary inflammatory response of injured spinal cord shortly after SCI, and EFS treatment could be a candidate for SCI therapy.