Efficient phase aberration compensation for digital holographic microscopy based on aberration-oriented phase unwrapping

Abstract

In digital holographic microscopy, the phase map of the sample can be severely distorted by phase aberration, which is caused by the introduction of the microscopy imaging system and the misalignment of its elements. In this study, we propose an automatic compensation method based on noncontinuous-path phase unwrapping and least-squares fitting. The method preferentially and integrally unwraps the large-area aberration term with low dynamic range. Accurate fitting is achieved without the need for background segmentation or complex optimization. To the best of our knowledge, this is the first study to focus on the sequence of unwrapping in phase aberration compensation. Numerical simulations demonstrate that the proposed method can compensate not only for tilt and spherical aberration but also for high-order and cross-term aberration. Experiment results show that the method has significant advantages in terms of speed and robustness.