Phase aberration compensation for digital holographic microscopy based on geometrical transformations

Abstract

In digital holographic microscopy (DHM), the use of microscope objective always introduces a phase aberration and thus affects the retrieved phase. To address this problem, we present a simple phase aberration compensation method in DHM based on geometrical transformations. After doing the geometrical transformations (including reflection, rotation of and transpose) for the unwrapping phase (+1 order) of the original hologram, we could get a transformation phase. By subtracting the transformation phase from the unwrapped phase (+1 order) of the original hologram, the parabolic phase aberration is compensated. The fully automatic and effective performance makes the proposal available for single-shot quantitative phase imaging without complex numerical fitting procedures or prior knowledge of the specimen and system. A series of simulations and the experiment results of the micro-lens array, phase plate and cells demonstrate the feasibility and effectiveness of the proposed method.