Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

Monika Kamińska,Alina Trejgell,Marcin Gołębiewski,Andrzej Tretyn

doi:10.1007/s11240-017-1343-z

Monika Kamińska, Alina Trejgell + Show 2 more

Open Access

https://doi.org/10.1007/s11240-017-1343-z

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Abstract

The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum, critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regrowth of shoot tips. The results indicated that synseeds obtained from shoot tips of T. pieninicum can be stored at 4 °C even for 12 months without subculture. The light is a stress factor during storage what was manifested by numerous necrosis and decreased shoots ability to proliferate in optimal growth conditions in 1st subculture. Additionally our results showed that the storage does not produce genetic variation at the resolution provided by the flow cytometry and RAPD analysis.

Highlights

The world’s biodiversity is declining at an unprecedented rate every year
Synthetic seeds of T. pieninicum showed high visual rate immediately after encapsulation but shoot tips lost their viability over time under optimal conditions
Cold storage of T. pieninicum was preceded by encapsulation of shoot tips isolated from the cluster, but the isolation and preparation of artificial seeds were stress factors that probably led to abscisic acid (ABA) synthesis in isolated tissue, which inhibited the conversion of synseeds after direct transfer to optimal conditions

Summary

Introduction

The world’s biodiversity is declining at an unprecedented rate every year. Over 5000 of plant species are listed as critically endangered (IUCN 2017). Conservation in the field shows major drawbacks (e.g. requirement of a large amount of area, exposure to disease or herbivores attack), which limit its efficacy and threaten the safety of plant genetic resources conserved in this way (Engelmann 2011). In vitro technique enables efficient short- or long-term storage, especially useful for the biodiversity protection by conservation of endangered species genome. In vitro micropropagation needs subcultures after each 4 weeks fraught with the risk of contamination and loss of culture. Slow growth procedures allow plant conservation without passages even for few years, depending on the species (Cruz-Cruz et al 2013)

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