Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions

Monika Kamińska,Jacek Kęsy,Alina Trejgell

doi:10.1007/s11240-020-01924-0

Abstract

Taraxacum pieninicum Pawł. is listed as critically endangered species, for which currently applied protection methods are insufficient. The aim of this study was to investigate the possibility of T. pieninicum storage in the form of synthetic seeds under slow-growth conditions in combination with ABA treatment, as one of the ex situ protection methods of this species. The obtained results indicated that darkness was much more favorable condition for synseed storage and did not generate additional stress during cold exposure in contrast to the light conditions. The preculture of shoot tips on the medium supplemented with ABA led to a decrease in the shoots proliferation rate and inhibition of their growth. ABA clearly inhibited growth of the encapsulated shoot tips also during cold storage. Biochemical parameters showed that ABA effectively reduced the negative effect of the cold stress, what was found on the basis of analyzes of H2O2 and TBARS levels in the stored material. Moreover, synseeds stored under light conditions and treated with ABA exhibited decreased level of endogenous jasmonic acid what indicated interaction between those two phytohormones at a low temperature. The study also demonstrated that in vitro culture, cold storage and ABA treatment had no effect on the flowering process of this species after acclimatization to ex vitro conditions.

Highlights

Communicated by Maurizio Lambardi.Electronic supplementary material The online version of this article contains supplementary material, which is available to authorized users.Taraxacum pieninicum belongs to the Asteraceae family and is a stenochoric species, occurring in only one site in the Central Pieniny in the Western Carpathians (Poland) (Piękoś-Mirkowa and Mirek 2009)
We have shown the possibilities of storing T. pieninicum synseeds and the usage of jasmonic acid as a growth retardant (Kamińska et al 2018a, b)
The aim of this research was to assess the effect of abscisic acid on the condition of plants obtained after long-term storage of Taraxacum pieninicum synthetic seeds

Summary

Introduction

Taraxacum pieninicum belongs to the Asteraceae family and is a stenochoric species, occurring in only one site in the Central Pieniny in the Western Carpathians (Poland) (Piękoś-Mirkowa and Mirek 2009). This species is considered to be probably the oldest endemic species in Pieniny Mts. from the Paleogene (Puchalski et al 2014). As the only species of the Taraxacum genus, it does not produce sesquiterpene lactones, such as taraxic acid and triterpenes, commonly referred to as taraxacin in older literature, which gives the leaf a bitter

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