Abstract
1. 1. The effects of several natural and synthetic nutrient media were tested on the DNA and protein-glycine contents of 11–13-somite chick embryos explanted 8 or 24 hours. 2. 2. Marked increases in both substances were obtained with explants on media containing the natural egg nutrient. Explants including more extensive areas of the extraembryonic membranes showed larger increases on such media than explants with smaller extraembryonic membranes. 3. 3. A glucose-containing medium promoted a large increase in DNA and a small increase in protein-glycine only in explants with large extraembryonic membranes. Other synthetic media had no effect on the protein-glycine content and promoted only a small increase in DNA. 4. 4. Under explantation conditions, with rapidly increasing protein-glycine contents, incorporation of glycine- 1-C 11 led to a small increase in the specific activities of the protein-glycine or protein-nitrogen. Under conditions of slow growth, a much greater increase in the specific activities was found to occur. This is interpreted to indicate utilization of different types of precursors for protein formation under conditions of rapid and slow growth.
Published Version
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