Effects of water extract of Salvia miltiorrhiza on superoxide dismutase, malondialdehyde and total antioxidative capacity in rats exposed to cadmium

Abstract

Objective To investigate the effect of water extract of Radix Salvia miltiorrhiza on superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidative capacity (T-AOC) in rats exposed to cadmium, to explore the protective effect of water extract of Radix Salvia miltiorrhiza on renal injury in rats exposed to cadmium. Methods 20 female Wistar rats and 20 male Wistar rats were randomly divided into blank control group (n=8) and model group (n=32). The rats were conventionally bred, the blank control group was intraperitoneally injected saline 5mL·kg-1·d-1, the model group was given 2.8mg·kg-1·d-1 of cadmium chloride solution by intraperitoneal injection.After the success in modeling, the model group was divided into administration group (salvia extract high dose 2.7g·kg-1·d-1, low dose of water extract of Salvia 1.35g·kg-1·d-1), negative control group, positive control group.After 4 weeks to experiment in the drug group, the water extract of Salvia miltiorrhiza were intragastricly administered, the positive control group using sodium selenite solution 0.05mg·kg-1·d-1 gavage, negative control group and the control group were given the same volume of saline water 2.7mL·kg-1·d-1, respectively.Each group was treated for 10 weeks.At the end of the experiment, the serum was collected to detect the serum SOD, MDA and T-AOC. Results Water extract of Salvia miltiorrhiza could make dye CD rats body weight growth faster, the serum SOD activity decreased significantly, serum MDA increased greatly, to enhance the serum total antioxidant capacity. Conclusion Water extract of Salvia miltiorrhiza has obvious antioxidative effect, it can significantly reduce swelling of renal tubular epithelial cells, improve renal function. Key words: Aqueous extract of Salvia miltiorrhiza; Cadmium; Antioxidant capacity; Renal injury