Effects of trichotecene mycotoxins on gene expression and activity of glutathione redox system in common carp (Cyprinus carpio L.)

Abstract

Event Abstract Back to Event Effects of trichotecene mycotoxins on gene expression and activity of glutathione redox system in common carp (Cyprinus carpio L.) Csilla Pelyhe1*, Benjámin Kövesi1, Erika Zándoki2, Balázs Kovács3, Miklós Mézes1 and Krisztián Balogh1 1 Szent István University, Department of Nutrition, Hungary 2 Hungarian Academy of Sciences, Kaposvár University, Mycotoxins in the Food Chain Research Group, Hungary 3 Szent István University, Department of Aquaculture, Hungary The purpose of present study was to evaluate the short- and long-term effects of T-2 toxin and deoxynivalenol (DON) in common carp. Short-term effect was evaluated using single oral doses of DON or T-2 toxin of 0.25, 0.50 or 3.00 mg/kg b.w.. Experimentally contaminated or control complete feed was given by gavage directly into the stomach. Hepatopancreas samples were taken from 6 carps of each group at every 8th hour during a 24-hour long experimental period. The long-term effect was evaluated in 4 weeks in a feeding trial using 0.05 mg T-2 toxin/kg b.w. and 0.06 mg DON/kg b.w., where hepatopancreas samples were taken from 6 carps of each group weekly. Reduced glutathione (GSH) concentration and activities of glutathione-peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) were measured. In addition, expressions of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) were analyzed. Lipid peroxidation was followed by the determination the amount of conjugated dienes (CDs) and -trienes (CTs) and malondialdehyde (MDA). Short-term exposure showed that both of the investigated mycotoxins caused rapid changes in the lipid peroxidation processes in the hepatopancreas, but the emerging free radical burden quickly activated the glutathione redox system, so the end-product of the lipid peroxidation (MDA) did not elevate significantly in the 24-hour period. Expression of the genes decreased after 8 hours and later increased during the 24-hour period. As effect of long-term exposure, the glutathione redox system showed decreased levels in T-2 toxin treated group, but the markers of lipid peroxidation processes did not elevate. Expression of the investigated genes showed dual response during the experiment. The results revealed that glutathione redox system eliminated effectively the peroxidative effect of the investigated mycotoxins in the applied doses and periods. Acknowledgements The research was supported by the National Scientific Research Fund (OTKA PD104823). Keywords: T-2 Toxin, Deoxynivalenol (DON), Antioxidant Enzymes, common carp, Glutathione redox system, gpx4 genes, Gene Expression Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Oral Presentation Topic: Physiology, Behavior and Toxicology Citation: Pelyhe C, Kövesi B, Zándoki E, Kovács B, Mézes M and Balogh K (2015). Effects of trichotecene mycotoxins on gene expression and activity of glutathione redox system in common carp (Cyprinus carpio L.). Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.fmars.2015.03.00028 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Oct 2015; Published Online: 04 Nov 2015. * Correspondence: Miss. Csilla Pelyhe, Szent István University, Department of Nutrition, Gödöllo, 2100, Hungary, pelyhe.csilla@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Csilla Pelyhe Benjámin Kövesi Erika Zándoki Balázs Kovács Miklós Mézes Krisztián Balogh Google Csilla Pelyhe Benjámin Kövesi Erika Zándoki Balázs Kovács Miklós Mézes Krisztián Balogh Google Scholar Csilla Pelyhe Benjámin Kövesi Erika Zándoki Balázs Kovács Miklós Mézes Krisztián Balogh PubMed Csilla Pelyhe Benjámin Kövesi Erika Zándoki Balázs Kovács Miklós Mézes Krisztián Balogh Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.