Effects of therapeutic doses of heparin on MSC functional activity in modeling the mechanisms of osteointegration under in vitro cultivation

Abstract

The use of artificial materials in regenerative medicine is accompanied by the development of an inflammatory response and the initiation of blood clotting due to the contact of the implant with the soft tissues of the body. All this leads to the formation of thrombosis of the major and main arteries and the development of serious complications. To prevent the development of postoperative pathological conditions caused by hypercoagulable syndrome, classical therapeutic strategies with anticoagulants (mainly heparin) are used. However, the described treatment tactics lead to an interruption of the migration and adhesion processes of mesenchymal stem cells (MSC), which negatively affects the callus formation and the processes of osseointegration of the implant. The aim of this study was to investigate the effect of a suspension of HA nanoparticles in the presence of heparin in a gradient of therapeutic concentrations on migratory and proliferative activity of MSCs in human adipose tissue under in vitro cultivation conditions. Methods. To evaluate the migration and proliferation potential of MSC in the presence of HA nanosuspension and/or heparin, an electrode system for continuous observation — xCELLigence ® RTCA DP was used. Results. A significant decrease in the migratory activity and proliferative potential of MSC was observed under the conditions of their co-cultivation with HA nanosuspension in the presence/absence of heparin (0.5–1 IU/ml). onclusion. The results of the study may serve as a prerequisite for the development of new therapeutic approaches for the use of heparin in surgical patients at high risk of postoperative thrombosis after osteosynthesis.