Abstract
Many workers have shown the depletion of synaptic vesicles following nerve stimulation but there is a discrepancy among data regarding the appearance of coated vesicles attendant on the decrease in the number of synaptic vesicles (Heuser and Reese, 1973; Ceccarelli et al., 1973; Pysh and Wiley, 1974). The present experiment was an attempt to investigate the effect of temperature on stimulation dependent coated vesicle formation.Dissected cat superior cervical ganglia were incubated in Krebs solution at 10°C and 37°C respectively. Preganglionic nerves were stimulated with the electrical parameters consisting of 1 msec square pulses of 20 V at 10 Hz for 30 min. After stimulation the ganglia were fixed with 2 % 0s04 (lh). To examine the participation of the coated endocytosis on recovery processes some ganglia were stimulated at 37°C for 30 min and then rested either at 37°C or 10°C for 5 and 15 min before fixation. The fixed material were then processed for electron microscopy with conventional method consisting of uranyl acetate block stain (30 min), dehydration with ethanol series, and embedding in Epon 812.
Published Version
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