Effects of sevoflurane on expression of heparanase and fascin in lung carcinoma cells of mice

Abstract

Objective To evaluate the effects of sevoflurane on the expression of heparanase (HPA) and fascin in lung carcinoma cells of mice. Methods Mouse LLC cells were inoculated in the culture plate.After being cultured for 24 h, the cells were equally and randomly divided into 4 groups using a random number table: control group (group CC) , 1% sevoflurane group (group Sev1) , 2% sevoflurane group (group Sev2) , and 3% sevoflurane group (group Sev3) . Cells in Sev1-3 groups were exposed to 1%, 2% and 3% sevoflurane, respectively, for 4 h, while cells in group CC were not exposed to sevoflurane, and all the cells were then cultured for another 24 h in an incubator.The invasion of cells was determined by Transwell invasion assay, and the invaded cells were counted.The migration of cells was determined by wound healing assay, and cell migration rates were calculated.The expression of HPA and fascin in cells was detected by Western blot. Results Compared with group CC, the number of invaded cells and cell migration rates were gradually decreased, and the expression of HPA and fascin was gradually down-regulated with increasing concentrations of sevoflurane in Sev1-3 groups. Conclusion The mechanism through which sevoflurane inhibits the metastasis of mouse lung carcinoma cells is associated with down-regulated expression of HPA and fascin. Key words: Anesthetics, inhalation; Lung neoplasms; Neoplasm metastasis; Heparin lyase; Cell movement