Effects of sevoflurane on invasion and migration of mouse lung cancer cells induced by hypoxia

Abstract

Objective To evaluate the effects of sevoflurane on invasion and migration of mouse lung cancer cells induced by hypoxia. Methods Mouse Lewis lung cancer cells were inoculated in the culture plate.After being cultured for 24 h, the cells were randomly divided into 3 groups(n=18 each)using a random number table: control group(group C), hypoxia group(group H)and hypoxia+ 2% sevoflurane group(group HS). Cells were exposed to 95% air-5%CO2(2 L/min)for 4 h in group C. Cells were exposed to 94% N2-5%CO2-1% O2 for 4 h in group H. In group HS, cells were exposed to 2% sevoflurane and 94% N2(2 L/min)for 4 h. The invasion of cells was determined by Transwell assay, and the invaded cells were counted.The migration of cells was evaluated by wound healing assay, and cell migration rates were calculated.The expression of Beclin 1 and LC3Ⅱ protein in cells was detected by Western blot. Results Compared with group C, the number of invaded cells and cell migration rates were significantly increased, and the expression of Beclin 1 and LC3Ⅱ was up-regulated in H and HS groups.Compared with group H, the number of invaded cells and cell migration rates were significantly decreased, and the expression of Beclin 1 and LC3Ⅱ was down-regulated in group HS. Conclusion Sevoflurane can inhibit the invasion and migration of mouse lung cancer cells induced by hypoxia, and inhibition of autophagy is involved in the mechanism. Key words: Anesthetics, inhalation; Lung neoplasms; Cell hypoxia; Neoplasm invasiveness; Neoplasm metastasis