Abstract
To investigate the effect of Fibrillin 2 (FBN2) expression on the invasion and migration of lung cancer cells, and the underlying mechanism. Protein and mRNA expressions of FBN2 were assayed. The relationship between FBN2 protein expression and clinical characteristics of lung cancer patients was analyzed. Correlation between FBN2 expression level and patient survival time was analyzed. Moreover, the mRNA and protein expression of FBN2 in lung cancer cells and human normal lung epithelial cells were assayed. After constructing low-expressing FBN2 cells, the cell proliferation, clone formation, migration and invasion capabilities were tested. Lung cancer cells proliferation with low FBN2 expression in nude mice was measured with a nude mouse tumorigenic experiment. The mRNA and protein expressions of FBN2 in lung cancer tissues were significantly higher than those in para-cancerous tissues (p<0.05). FBN2 protein expression was significantly correlated with TNM stage, lymph node metastasis and histological type (p<0.05). Survival time was markedly reduced in patients with high FBN2 expression (p<0.001). The expressions of FBN2 mRNA and protein were markedly higher in lung cancer cells than in human normal lung epithelial cells. The proliferation, migration and invasion of lung cancer cells were significantly inhibited by FBN2 knockdown. The FBN2 knockdown significantly inhibited the protein expressions of p-FAK, p-MEK and p-ERK. FBN2 is highly expressed in lung cancer tissues, and as an oncogene, it affects the pathogenesis of lung cancer. The knockdown of the expression of FBN2 significantly inhibits the proliferation, invasion and migration abilities of lung cancer cells.
Highlights
Lung cancer is one of the most malignant tumors with the highest cancer-related mortality in the world, and it is a serious threat to the health of Chinese people [1, 2]
Immunohistochemistry was used to analyze the protein expressions of Fibrillin 2 (FBN2) in 97 cases of lung cancer tissues and the results showed that FBN2 protein was highly expressed in lung cancer tissues
These results indicate that the FBN2 gene was highly expressed in lung cancer tissues, suggesting that it may be involved in the pathogenesis of lung cancer
Summary
Lung cancer is one of the most malignant tumors with the highest cancer-related mortality in the world, and it is a serious threat to the health of Chinese people [1, 2]. Lung cancer is divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). It has been reported that NSCLC, which is more common than SCLC, accounts for about 80% of all cases of lung cancer [3]. There are no specific clinical symptoms at the early onset of lung cancer. Most patients are usually diagnosed with the disease at the advanced stages. This is a serious threat to the long-term survival of patients. It is important to identify early markers of lung cancer so as to improve the early diagnosis of the disease
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