Effects of Power Frequency Magnetic Fields Acute Exposure on Growth and Apoptosis of PC12 Cells

Abstract

PC12 cells were cultured in vitro,then exposed to 50 Hz and 100 μT extremely low frequency electromagnetic fields for 24 h.The proliferation activity,cell cycle and apoptosis was detected respectively by methyl thiazolyl tetrazolium colorimetry,flow cytometry and acridine orange/ethidium bromide,double label immunofluorescence.It was found that: 1) After exposure,proliferation activity was decreased at 0 h(P0.01),but increased at 8 h(P0.05) and 12 h(P0.01),and there were no significant changes at 4 h.2) After exposure,G0/G1 cells were increased(P0.01) and S cells were decreased(P0.05) at 0 h,G2/M cells were increased(P0.05) at 6 h,G0/G1 cells were decreased(P0.01) and S cells were increased(P0.05) at 12 h,there were no significant changes at 24 h.3) The apoptotic rates were increased significantly at 12 h(P0.05),18 h(P0.01) and 24 h(P0.01) during exposure,and 6 h,12 h,18 h and 24 h after exposure(P0.01).The results indicated that 50 Hz and 100 μT extremely low frequency electromagnetic fields exposure for 24 h can change the proliferation activity and cell cycle of PC12 cells and increase cell apoptosis.