Abstract
The quenching by subphase iodide of pyrene covalently bound to amphiphatic molecules in spread monolayers has been measured. The probes employed were 2-(6-pyreneylhexanoyl)-3-palmitoyl-sn-glycero-1-phosphorylcholine, 10-(6(8)-octadecylpyrenyl) decanoic acid and 1-pyrenedocosanoic acid; the host lipids utilized were dioleoylphosphatidylcholine (DOL) and dipalmitoylphosphatidylcholine (DPL). It has been observed in all cases that pyrene fluorescence is significantly quenched in the uncompressed layer and that this quenching diminishes markedly as the surface pressure is increased over the liquid expanded region. Beyond this range a plateau in the fluorescence decay is reached in DOL, while in DPL changes in quenching are related to phase transitions. These findings indicate significant probe-water interaction at low surface pressure. Residual iodide quenching in DOL above 10 dyn cm −1 indicates some continued water-probe contact which may be due to the liquid character of the host layer and the double-bond character of the lipid while the very small residual quenching in DPL suggests tight packing in the non-liquid phases.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.