Abstract
Eggs of zebrafish ( Danio rerio) were exposed to microcystin-LR at concentrations of 0.5, 5 and 50 μg litre −1 from blastula stage up to the end of embryonic development. No effects were observed during this period. After termination of exposure and transfer into microcystin-LR-free water, the evaluation at the end of the larval period showed decreased survival at 5 and 50 μg litre −1 microcystin-LR. Furthermore, concentrations of 0.5, 5 and 50 μg litre −1 microcystin-LR retarded larval growth. Far more pronounced effects occurred following exposure with various aqueous cyanobacterial crude extracts from field samples and batch cultures: gross malformations and high mortalities during embryonic development were observed.
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