Effects of matrine on apoptosis and nuclear factor kappaBp65 activity of HL-60 cells induced by pirarubicin

Abstract

Objective To explore the effects of matrine on apoptosis and nuclear factor(NF)-κBp65 activity of HL-60 cells induced by pirarubicin(THP). Methods Effects of the apoptosis: the HL-60 cells in blank control group were cultured 14 hours with RPMI 1640; groups of different concentration drugs: matrine: 0.1 μmol/L, 1.0 μmol/L, 10.0 μmol/L and THP: 1.0 μmol/L, 10.0 μmol/L, 100.0 μmol/L.The apoptosis of HL-60 cells were tested by fluorescence double colour painting and flow cytometry(FCM). The NF-κBp65 activity of HL-60 cells were determined by FCM. Results The rates of apoptosis of HL-60 cells were (7.14±2.95)%, (12.34±2.55)%, (19.3±2.31)% and (31.78±4.31)%, (47.25±5.27)%, (56.49±1.59)% in matrine 0.1 μmol/L, 1.0 μmol/L, 10.0 μmol/L and THP 1.0 μmol/L, 10.0 μmol/L, 100.0 μmol/L groups, compared to blank control group(6.46±1.45)%, there were significant difference (F=257.72, 677.19; all P<0.001); (70.17±5.68)% in matrine 0.1 μmol/L + THP 1.0 μmol/L group[vs the THP 1.0 μmol/L group (31.78±4.31)%, (t=38.94, P<0.001)]. The rates of NF-κBp65 activity of HL-60 cells in matrine 0.1 μmol/L, 1.0 μmol/L, 10.0 μmol/L and THP 1.0 μmol/L, 10.0 μmol/L, 100.0 μmol/L groups were(8.34±1.52)%, (7.11±1.29)%, (4.78±0.31)% and (16.21±1.20)%, (23.98±3.21)%, (32.44±2.89)%, with the control group( 8.44±2.20)%, there were significant difference(F=65.35, P<0.001; F=674.11, P<0.001); (12.01±2.27)% in matrine 0.1 μmol/L + THP 1.0 μmol/L group [with (16.21±1.20)% of THP 1.0 μmol/L group(t=21.42, P<0.001)]. Conclusions The apoptosis of HL-60 cell is induced by matrine and pirarubicin in a dose-dependent manner.NF-κBp65 activity of HL-60 cell is inhibited by matrine and increased by pirarubicin in a dose-dependent manner.Matrine can enhannce the effect of induction by pirarubicin of apoptosis of HL-60 cell, and decrease activity of NF-κBp65 by pirarubicin. Key words: Matrine; Pirarubicin; Drug combination; Apoptosis; Nuclear factor-κBp65 activity