Effect of Matrine on the expression of X-linked inhibitor of apoptosis protein in human rhabdomyosarcoma RD cells

Abstract

Objective To investigate the effect of Matrine on the expression of X-linked inhibitor of apoptosis protein (XIAP) in human rhabdomyosarcoma RD cells in vitro. Methods Cultured human rhabdomyosarcoma RD cells were divided into Matrine intervention groups (0.5 g/L, 1.0 g/L and 1.5 g/L) and a control group.The proliferation-inhibition rates in RD cells treated with different concentrations of matrine were detected by methylthiazolyl blue colorimetric assay.Flow cytometry analysis was performed for the apoptosis rates of RD cells.Reverse transcription-polymerase chain reaction analysis was used to measure the XIAP mRNA expression. Results There was a significant difference in the proliferation-inhibition rates [0.5 g/L Matrine group: (15.84±2.58)%, 1.0 g/L Matrine group: (23.13±4.19)%, 1.5 g/L Matrine group: (30.32±3.02)%, and the control group: (8.92±1.23)%]; apoptotic rates [0.5 g/L Matrine group: (12.33±1.15)%, 1.0 g/L Matrine group: (16.67±0.99)%, 1.5 g/L Matrine group: (25.33±1.91)%, and the control group: (9.47±0.96)%]; XIAP mRNA expression(0.5 g/L Matrine group: 0.633±0.046, 1.0 g/L Matrine group: 0.441±0.055, 1.5 g/L Matrine group: 0.326±0.065, control group: 0.794±0.029)in RD cells among 0.5 g/L, 1.0 g/L, 1.5 g/L Matrine groups and the control group (F=14.15, 83.37, 50.57, all P<0.05). The proliferation-inhibition and apoptotic rates in RD cells were gradually increased with the increasing Matrine concentration.The expression of XIAP mRNA was significantly decreased in different Matrine groups compared with the control group, exhibiting a dose-dependent manner. Conclusions Matrine can inhibit the proliferation of RD cells and induce the apoptosis in a dose-dependent manner, which may be related to the down-regulated XIAP mRNA. Key words: Human rhabdomyosarcoma RD cell; Matrine; X-linked inhibitor of apoptosis protein