Effects of matrine on human neuroblastoma cell SH-SY5Y

Abstract

Objective To investigate the effect of matrine on human neuroblastoma cell SHSY5Y(NB SH-SY5Y). Methods The cells at logarithm growth phase were chosen and randomly divided into control group without matrine and four groups of different matrine concentrations,in which matrine concentrations were 0. 5 mg/ml, 1.0 mg/ml,2. 0 mg/ml and 3. 0 mg/ml, respectively. Five duplicating holes were set up on each group. After the cells were cultured for 48 hours, the proliferation inhibition rate was detected by MTT assay,the apoptosis rate by flow cytometry (FCM),and the expression of TrkA mRNA by RT-PCR. Statistical analysis was performed using SPSS16. 0 software for one-way ANOVA. Results The proliferation inhibition ratios of all the groups were: (7. 25 ± 1.55)％, (12. 56 ± 1.94) ％, (17. 22 ± 0. 90) ％, (22. 39 ± 0. 78) ％ and (25.35 ± 1.38) ％ ,respectively. The apoptosis ratios were (5.30±0. 60) ％,(10. 04± 1.18) ％,(14. 34± 1.79) ％,(21.36±0. 96) ％ and (24. 80 ± 1.53) ％ ,respectively; The expression of TrkA mRNA were 0. 502 ± 0. 043,0. 584 ± 0. 032,0. 644 ± 0. 052,0. 836 ± 0. 056 and 0. 948 ± 0. 030, respectively. Significant differences were found between all the groups (P＜0. 05). Conclusions Matrine could restrain the NB SH-SY5Y cells proliferation, induce the cells apoptosis, and increase the expression of TrkA mRNA in vitro. Matrine may be used for the novel treatment of neuroblastoma. Key words: Matrine; Neuroblastoma; Cell proliferation; Apoptosis