Effects of intraneural injection of Ricinus communis agglutinin-60 into rat vagus nerve.

Abstract

The dorsal motor nucleus (DMN) of the rat was studied at various survival periods following an intraneural injection of Ricinus communis agglutinin-60 (RCA-60) into the vagus nerve at the mid-cervical region. No obvious structural changes were noted in the DMN 2 and 4 days after the injection of RCA-60. At 5 and 6 days after the RCA-60 injection, the larger neurons (measuring 19 X 12 microns) in the DMN underwent chromatolytic degeneration whereas the smaller ones (measuring 10 X 6 microns), characterized by their infolded nuclei, remained unaffected. The majority of the degenerating DMN neurons became pale and crenated in outline. Other structural changes included swollen mitochondria with disrupted cristae and profiles of rough endoplasmic reticulum denuded of ribosome particles. A few of the degenerating neurons became extremely condensed and darkened. Axon terminals which showed synaptic contacts with these cells remained normal. Both pale and darkened degenerating dendrites, derived from the degenerating neurons, were present in the neuropil. In addition to these, degenerating axon terminals with clumping or swelling of synaptic vesicles were also present. They were presynaptic to dendrites of various sizes. Massive infiltration of mononuclear cells occurred in the DMN. These cells reached the DMN by diapedesis and were actively engaged in the phagocytosis of degenerating neuronal elements. While most of the invading cells transformed into active neuronal macrophages, some of them eventually died in the neuropil of the DMN. Light microscopic study by Fink-Heimer's method for degenerating fibres and terminals revealed their distribution to the DMN, nucleus of the tractus solitarius, nucleus commissuralis, dorsolateral and lateral part of the hypoglossal nucleus and the area postrema. It was concluded from this study that RCA-60, when injected into the cervical vagus was retrogradely transported to the cell body of the DMN neurons of the larger category. The selective destruction of the DMN neurons by RCA-60 elicited a massive infiltration of mononuclear cells which gave rise to the neural macrophages. The RCA-60 injected also killed the vagal sensory neurons as demonstrated by the numerous degenerating fibres and axon terminals in the DMN which would represent their central processes.