Effects of Different Growth Regulating Substance on the Actinidia deliciosa Leaves Excised Direct Regeneration

Abstract

Using the leaves of test-tube seedlings of Actinidia deliciosa as expiants, the effect of different growth regulating substance on the Actinidia deliciosa leaves excised regeneration system was researched, including the differentiation of adventitious buds, the regeneration and rooting of regenerated shoots. The results indicated that the maximum adventitious buds regeneration frequency achieved 76.6% on the medium of Murashige and Skoog medium(MS) containing 5.0 mg·L−1 6-benzylaminopurine(6-BA) and 0.2 mg·L−1 1-naphthaleneacetic acid (NAA); the most suitable medium for shoot multiplication was MS with 2.0 mg·L−1 6-BA, 0.2 mg·L−1 NAA and 0.1 mg·L−1 gibberellin acid(GA3) and the multiplication coefficient was up to 4.76; 1/2MS with 0.7 mg·L−1 1h-indolo-3-butanoic acid(IBA) was the most suitable for the rooting of regenerated shoots, and the rooting rate was 100%.