Abstract
Broussonetia papyrifera is an important ecological and economic tree species. The sexual reproduction of B. papyrifera not only has a low germination rate, but also requires high environmental conditions. Therefore, asexual propagation using tissue culture can effectively improve the propagation efficiency of B. papyrifera. In this study, the leaves and budded shoots of B. papyrifera were used as explants, and different concentrations of plant growth regulators were added to Murashige and Skoog medium (MS) to establish a suitable system for explant callus formation, adventitious buds differentiation and rooting. The results showed that MS + 0.50 mg/L naphthaleneacetic acid (NAA) + 0.25 mg/L 6-benzyladenine(6-BA) and MS + 0.25 mg/L NAA + 0.50 mg/L 6-BA were the best mediums for rapid callus induction from leaf explants and shoot explants, respectively. The best medium combination for shoot differentiation and proliferation was MS + 0.05 mg/L NAA + 0.50 mg/L 6-BA, and the high propagation coefficient could also promote adventitious bud growth. The best rooting medium in the establishment of B. papyrifera tissue culture was MS + 0.25 mg/L NAA. Under this condition, the average rooting numbers of leaf explants and shoot explants were 1.71 and 13.86, respectively. In addition, the best transplanting substrate was a mixture of soil:perlite:vermiculite (20:1:1), and the survival rate was 91.1%. This study established a propagation system in vitro culture of B. papyrifera, and provided a reference for tissue culture of other woody plants.
Highlights
Plant tissue culture is the fastest and most effective way to produce virus-free plants, which can solve the problem of an insufficient supply of plants
The results showed that the diameter of shoot explants callus reached the maximum value of 1.3 ± 0.15 cm when the naphthaleneacetic acid (NAA) concentration was 0.25 mg/L and the
This study showed that the effects of 6-BA and NAA on shoot and leaf explants were not completely the same
Summary
Plant tissue culture is the fastest and most effective way to produce virus-free plants, which can solve the problem of an insufficient supply of plants. Plant tissue culture technology is widely used in various plants to promote factory production of plants and protect endangered plants, especially in woody plants [1,2]. Tissue culture technology can preserve the fine genetic traits of plants [3]. Plant growth regulators play a very important role at various stages in plant tissue culture [4]. In the rooting experiment of Coleonema album Thunb. Was 42.5% higher than that without any plant growth regulators [5]. The concentration of growth regulators was important for plant growth [6]. In an experiment on the effect of plant growth regulators concentration on the rooting of Magnolia lucida B.
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