Abstract
目的: 探讨丹珍头痛胶囊对中枢敏感介导的慢性偏头痛大鼠的干预作用。方法: 将60只SD大鼠随机分为正常组、模型组、盐酸氟桂利嗪组(FNZ)、丹珍头痛胶囊(DZTT)高、中、低剂量组(1.0、0.5、0.25 g/kg)(n=10)。采用连续皮下注射硝酸甘油法制作慢性偏头痛大鼠模型,丹珍头痛胶囊给予大鼠灌胃治疗,每天1次,连续35 d。观测各组大鼠大体状态、耳红出现及消失时间、搔头次数、爬笼次数等疼痛行为学指标,测定眶周及足底的机械痛阈值,RT-PCR及Western blot技术分别检测三叉神经脊束核尾侧(TNC)组织中CREB、ERK的mRNA表达及CREB、pCREB、ERK、pERK蛋白表达水平。结果: 与正常组比较,模型组大鼠行为学评分明显升高,眶周及足底机械痛阈值逐日下降,TNC组织中CREB、ERK基因表达及CREB、pCREB、ERK、pERK蛋白表达水平均显著升高(P＜0.01);与模型组比较,DZTT、FNZ组大鼠疼痛症状明显缓解,疼痛行为学评分显著降低而眶周及足底机械痛阈值显著升高,TNC组织中CREB、ERK基因表达及CREB、pCREB、ERK、pERK蛋白表达水平均显著下降(P＜0.05,P＜0.01);与FNZ组比较,在给药第35日,DZTT高剂量组大鼠疼痛行为学评分、眶周及足底机械痛阈、TNC组织中CREB mRNA表达及ERK、pERK蛋白表达水平差异无显著性(P＞0.05)。结论: DZTT对中枢敏感介导的慢性偏头痛具有一定的治疗作用,抑制CREB、ERK的激活和磷酸化是其可能机制之一。.
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Schedule a callMore From: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
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