Effects of BaCl2 on slow vacuolar ion channels on radish by patch-clamp

Abstract

The effects of BaCl 2 on slow vacuolar (SV) currents of radish are studied by using the whole-vacuolar patch-clamp recording mode. The Ca 2+ -dependent SV channel can be activated by cytosolic Ca 2+ . When 1 mmol/L BaCl 2 is added into pipette solution, SV currents are suppressed remarkably. Then adding BaCl 2 of different concentrations into the bath solution, SV currents reflect different effects. The results show that BaCl 2 with a lower concentration (＜ 3 mmol/L) promotes the channel currents and the currents are saturated when BaCl 2 concentrations are between 1 mmol/L and 1 mmol/L, but BaCl 2 with higher concentration (≥ 3 mmol/L) inhibits SV currents.