Abstract
Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu 2+ on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu 2+ in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu 2+ significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu 2+ . These results show that there may be some Cu 2+ binding sites on SV channels and binding to which can change SV current’s characteristic.
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