Effects of avocado oil on kidney mitochondrial membrane potential of L‐NAME‐treated rats (953.4)

Abstract

Hypertension is an important risk factor for the development of terminal kidney disease. Mitochondria have been proposed as a novel target for the treatment of hypertensive complications because has been demonstrated that the prevention of mitochondrial dysfunctions through the improvement of redox status by antioxidant therapy ameliorates vascular dysfunction caused by angiotensin II. Recently, we have reported that avocado oil intake has beneficial effects in kidney mitochondria of diabetic rats by improving the function of the electron transport chain (ETC) and decreasing ROS generation. Since kidney mitochondrial dysfunctions in both diabetes and hypertension shares enhanced oxidative stress features and avocado oil is a rich source of antioxidants, we aimed to test the effects of 90‐days avocado oil supplementation on kidney mitochondrial membrane potential of L‐NAME‐treated rats, a model of hypertension. No significant differences were observed in the phases of hyperpolarization and depolarization of membrane potential when succinate plus rotenone or glutamate‐malate plus succinate were used as respiratory substrates. However, when glutamate‐malate was used alone, a severe inhibition of membrane potential of control and L‐NAME rats was observed, suggesting impairment in complex I function. These effects were partially prevented by avocado oil supplementation. Interestingly, a protective effect of similar magnitude was obtained when L‐NAME rats were treated with losartan, an AT‐1 receptor antagonist. These data suggest that avocado oil may protect from mitochondrial ETC dysfunction in hypertensive kidney by decreasing pro‐oxidative effects of angiotensin II.Grant Funding Source: Supported by CONACYT (130638 to CCR) and CIC‐UMSNH (16.12 to CCR) grants