Abstract
Oil extracted from cloves, Syzygium aromaticum, consists mainly of eugenol, which has been documented to affect protozoa attachment, viability and growth. Isoeugenol (sold under the trade name AQUI-S®) is a commercially available fish anaesthetic produced by isomerisation of eugenol. Both are anaesthetics commonly employed during the husbandry and euthanasia of Atlantic salmon, Salmo salar L., used for amoebic gill disease (AGD) research. Neoparamoeba perurans (the causative agent of AGD) trophozoites were exposed to clove oil at 10, 20, 40 and 80μLL−1 for 10min and 5, 10, 20 and 40μLL−1 for 120min; AQUI-S® at 5, 10, 20 and 40μLL−1 for 10min and 2.5, 5, 10 and 20μLL−1 for 120min There were no significant differences in viability and survival of trophozoites after exposure to the anaesthetics. When trophozoites were exposed to clove oil at 80μLL−1 for 10min there was significantly (P<0.05) more detachment from a plastic surface compared to the control. Continued use of clove oil and AQUI-S® at 40μLL−1 or less for up to 120min is unlikely to have a detrimental impact on amoebae that are isolated and collected from salmon with AGD to be used for downstream research such as projects involving disease challenges and in vitro screening of anti-N. perurans compounds. Statement of relevanceWe believe that the study has relevance because it establishes that there were no anti-amoeba properties of eugenol observed at concentrations commonly used and therefore interpretation of previous in vitro and in vivo work unlikely to be affected as will future work.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call