Abstract
A halotolerant and highly effective phenol-degrading strain, named as JDD1H, was isolated from soil contaminated by petroleum and identified as Rhodococcus sp. Phenol degradation by strain JDD1H in the presence of high salinity, especially the effect of exotic stimulants such as carbon source, nitrogen source and surfactant on phenol degradation was investigated. The results show that strain JDD1H could utilize phenol as sole carbon and energy source to grow in the presence of 5% NaCl with 16 h adaptive phase. The degradation efficiency of 150 mg/L phenol was up to 99.5% within 40 h. Lactose, cane sugar, glucose and mannitol in wide range of 100~2000 mg/L enhanced phenol degradation significantly. Organic nitrogen sources, as peptone and yeast extract, and inorganic nitrogen sources, as NH4Cl and (NH4)2SO4, accelerated phenol degradation. Tween-80 with concentration of 50-1000 mg/L restrained phenol degradation and Tween-80 of 1500-2000 mg/L could promote degradation.
Published Version
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