Optimization of Medium Composition for Inulin Fructotransferase (IFTase) Production by Nonomuraea sp.

Abstract

Inulin fructotransferase is an enzyme which converts inulin into difructose anhydride III (DFA III). Inulin fructrotransferase was produced by fermentation in medium containing various concentrations of inulin (10 g/L, 20 g/L) as carbon source, yeast extract (1 g/L, 2g/L) as organic nitrogen source and (NH4)2SO4 (2g/L, 5g/L) as inorganic nitrogen source using Nonomuraea sp. ID06-A0189. The aim of the study was to optimize the medium composition using various concentrations of carbon and nitrogen sources for inulin fructotransferase production by Nonomuraea sp. ID06-A0189, through shake flask system. Fermentation was conducted at 30 °C, pH 7.0 and agitated in 130 rpm for three days. The result showed that comparable with those found in another condition the highest enzyme activity was produced in medium using inulin as carbon source with concentration of 10 g/L, yeast extract as organic nitrogen source with concentration of 5 g/L and ammonium sulfate as inorganic nitrogen source with concentration of 2 g/L. Addition of inulin as a carbon source increased the volume of biomass by about 11.5-24 mL. The enzyme activity in the culture supernatant reached 25.3 U/mL after three days incubation.