Abstract
In recent years conducted studies showed that mycobacteria are the most resistant microorganisms to negative environmental factors, physical and chemical effects. One of the major issues of animal tuberculosis is the correct selection of effective disinfectants. The aim of our studies was to estimate the interaction of aldehyde disinfectant on animal tuberculosis agents. It was found that the disinfectant, contained glutaraldehyde and formaldehyde, exhibits bactericidal properties on M. bovis and M. avium , as confirmed by culture, molecular genetics and electronic-microscopic research methods. The disinfectant partially destroyed the integrity surface of cellular structures in M. avium . The cytoplasm acquires density and contains vacuoles and finely granular substance. The area of nucleoid is blurred. In M. bovis cells the preparation causes destruction of cell surface structures on different poles, leading to the release of the cytoplasm and consolidation of the nucleoid area. Biocidal activity of the aldehyde disinfection preparation consists in the destruction of Mycobacterium surface structures, formation of vacuoles and osmiophilic granules in the cytoplasm. The aldehyde disinfectant impacts on mycobacterial cells with the comprehensive irreversible changes in structural elements that lead to the complete destruction of microorganisms.
Highlights
According to the Bergey’s Manual of Systematic Bacteriology, mycobacteria belong to the order Actinomycetales family Mycobacterium and genus Mycobacteriaceae (Skerman et al, 1980)
Mycobacterium tuberculosis and atypical mycobacteria are highly adaptive due to its plasticity properties to the action of antibiotics and placed in ascending order: M. bovis, M. tuberculosis, M. avium, M. fortuitum, and the acquisition of a stable resistance change of Mycobacterium accompanied by some differential phenotypic properties (Dyachenko et al, 2009)
At the first step the bactericidal activity of the disinfectant was estimated by suspension on the test cultures of fast-growing atypical M. fortuitum with the concentration of 0.5, 1.0, 1.5, and 2.0% for the exposure of 1, 5, and 24 hours (Table 1)
Summary
According to the Bergey’s Manual of Systematic Bacteriology, mycobacteria belong to the order Actinomycetales family Mycobacterium and genus Mycobacteriaceae (Skerman et al, 1980). One of the issues that need to be resolved today is systematic evaluation of sensitivity of reference and circulating strains of microorganisms to disinfectants (Shkarin et al, 2008). It was found that epizootic culture of M. bovis is more resistant to disinfectants than the reference strain. The most stable to the bactericidal activity of disinfectants from various chemical groups are M. scrofulaceum, M. intracellulare, M. fortuitum and M. avium (Paliy, 2014). Mycobacterium tuberculosis and atypical mycobacteria are highly adaptive due to its plasticity properties to the action of antibiotics and placed in ascending order: M. bovis, M. tuberculosis, M. avium, M. fortuitum, and the acquisition of a stable resistance change of Mycobacterium accompanied by some differential phenotypic properties (Dyachenko et al, 2009)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
