Abstract
Effect of various nutritional supplementations on production of hyaluronic acid (HA) using Streptococcus equi subsp. zooepidemicus ATCC 39920 was evaluated at shake flask. Among the different carbon sources used, maltodextin showed HA of 0.9 g/L followed by 0.7 g/L for dextrose. L-arginine HCl at 2.0 g/L increased the yield of HA from 0.7 g/L to 0.85 g/L. L-arginine and L-glutamine showed the yield of HA of 0.7 g/L and 1.0 g/L, respectively at 2.0 g/L concentration. L-glutamine (2.0 g/L) and uridine (0.75 g/L) showed HA yield of 0.8 and 1.045 g/L, respectively. Addition of sodium glucuronate at 0.25 g/L concentration at 8 h, the yield of HA was increased from 0.7 g/L to 0.85 g/L. Pantothenic acid at concentration of 8.33 mg/L supplemented to the P10 medium, yield of 0.87 g/L of HA was obtained. Active dry yeast at 5.0 g/L increased yield of HA from 0.7 g/L to 0.85 g/L. Tween 80 at higher concentration reduced HA production. The addition of MgSO4.7H2O at 1.0 g/L improved the production of HA. Other metal ions like manganese, ferrous, copper, and zinc have no significant effect on HA production. Thus, it has been found that various nutritional supplements have effect on production of HA using S. equi subsp. zooepidemicus ATCC 39920
Highlights
Hyaluronic acid (HA) is a high molecular weight linear polysaccharide composed of repeating units of Dglucuronic acid and N-acetylglucosamine linked by β (13) and β (1-4) glycosidic bonds
We have investigated effect of various nutritional factors like amino acids (L-arginine HCl and L-glutamine), nutritional supplements to produce higher amounts of HA in Streptococcus equi subsp. zooepidemicus American Type Culture Collection (ATCC) 39920 at shake flask
S. equi subsp. zooepidemicus ATCC 39920 was obtained from ATCC and subsequently studied for their stability of HA production characteristics upon maintenance and storage
Summary
Hyaluronic acid (HA) is a high molecular weight linear polysaccharide composed of repeating units of Dglucuronic acid and N-acetylglucosamine linked by β (13) and β (1-4) glycosidic bonds. The commonly used strain for microbial HA production on an industrial scale is Streptococcus zooepidemicus, which synthesizes HA as the extracellular capsule (Duan et al, 2008). Much work such as improving the fermentation process (Duan et al, 2008; Liu et al, 2008b) adding lysozyme (Ogradowski et al, 2005), the alkaline-strategy (Liu et al, 2008a), adding hydrogen peroxide and ascorbate (Liu et al, 2009) and changing the medium composition (Rangaswamy and Jain 2008; Zhang et al, 2006) has been done to increase the production yield of HA in Streptococcus species. Many studies have been performed on HA production, but very few reports were published on study of different supplementation to increase the production of HA
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