Effect of TRPM7 on the proliferation and apoptosis in bladder cancer T24 cells

Abstract

Objectives To investigate the effects of transient receptor potential melastatin 7 (TRPM7) on the proliferation and apoptosis in T24 bladder cancer cell lines and the underlying molecular mechanisms.Methods Expression of TRPM7 mRNA and protein in T24 cell line were detected with RT-PCR and Western blot; channel blockers and gene silencing were used to block the function of TRPM7,MTT assay was used to detect cell viability,flow cytometry was used to analyze cell cycle distribution and apoptosis rate,Western blot was used to detect the expression of Cdk4,Cdk6 and Cyto C.Results RT-PCR and Western blot analysis confirmed over-expression of TRPM7 mRNA and protein in T24 cell lines ; after using gene silencing and channel blockers to block the function of TRPM7,the viability of T24 cell decreased by 56.48％ and 54.87％ respectively,T24 cells at G0 / G1 stage and the apoptosis rate increased significantly in a dose-dependent manner.Compared with the control group,the differences were statistically significant (p ＜ 0.05).Western blot showed that after blocking TRPM7 expression,Cdk4,Cdk6 in T24 cells decreased,while the expression of Cyto C increased.Conclusions TRPM7 ion channel can promote cell proliferation and inhibit cell apoptosis in T24 cells.This process may achieve by regulating the expression of Cdk4,Cdk6 and Cyto C.Blocking TRPM7 function,T24 can inhibit cell proliferation and induce apoptosis,TRPM7 may provide a new target for the clinical treatment of bladder cancer. Key words: Urinary Bladder Neoplasms ; Cell Proliferation ; Apoptosis