Effect of TRPM8 on proliferation and apoptosis of human bladder transitional cell carcinoma cell T24

Abstract

Objective To investigate the effect of down-regulation of transient receptor potential melastatin 8 (TRPM8) on the proliferation and apoptosis of human bladder transitional cell carcinoma cells T24. Methods shRNA targeting TRPM8 was designed and synthesized, and then transfected into the T24 cells via lipofectamine 2000 mediation. The proliferation and apoptosis of T24 cells were detected with methyl thiazolyl tetrazolium (MTT) assay and flow cytometry. Expression of extracellular regulated kinase(ERK), cyclin D1, and Bcl-2 were detected with Western blot. Results TRPM8-targeted shRNA down-regulated TRPM8 expression of T24 cells. MTT assay showed a significant acceleration of the proliferation of shRNA interference group compared to blank and control groups (P<0.01). Compared to control group, cell apoptosis rate was significantly higher in shRNA interference group (P<0.01). In addition, the expressions of PI3K, cyclin D1, and Bcl-2 were decreased in shRNA interference group. Conclusions Down-regulation of TRPM8 can induce inhibition of proliferation and promotion of cell apoptosis in human bladder transitional cell carcinoma cells T24 via regulating PI3K. It might be regarded as a novel target for clinical diagnosis and gene therapy of bladder cancer. Key words: Transient receptor potential channels/BI/PD; RNA interference; Urinary bladder neoplasms/TH/ME/PP; Cell proliferation/DE; Apoptosis/DE